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Application of ,nucleic acid, based techniques for ,detection, of ,Classical swine fever, virus: A comparative study Elina Khatoon*1, Nagendra Nath Barman2, Manab Deka3, Gitika Rajbongshi4, Kongkon Baruah5, Nipu Deka6, Durlav Prasad Bora7 1 & 3 Department of Biotechnology, Gauhati University, 781014, Assam
Classical swine fever, virus (CSFV) is the causative agent of ,Classical swine fever, which is a highly contagious disease affecting ,swine, and resulting in severe economic losses. In this study, we developed reverse transcription loopmediated isothermal amplification (RT-LAMP) assay targeting the 5’UTR gene for the ,detection, of CSFV. This amplification method can be obtained in 1 h under ...
site ,detection,; lateral flow dipstick; ,nucleic acid, . 32. ,detection,. 33. Introduction. 34 35 ,African swine fever, (ASF) is a highly contagious hemorrhagic disease of domestic pigs and wild boars. 36. ASF is caused by a large, complex double-stranded DNA virus, the ,African swine fever, virus (ASFV) . 37. The disease. shares similar clinical ...
African swine fever,: ,detection, and diagnosis 9 789251 097526. Cover photographs: ... 18 Haemorrhages in a pig with ,classical swine fever, (CsF) 24. vi ... eDTA Ethylenediaminetetraacetic ,acid, eFSA European Food Safety Authority eLISA Enzyme-linked immunosorbent assay
Classical swine fever, virus (CSFV) is the causa- tive agent of ,Classical swine fever, which is a highly contagious disease affecting ,swine, and resulting in severe economic losses. In this study, we developed reverse transcription loop- mediated isothermal amplification (RT-LAMP) assay targeting the 5’UTR gene for the ,detection, of CSFV.
Detection, of ,African swine fever,, ,classical swine fever,, and foot-and-mouth disease viruses in ,swine, oral fluids by multiplex reverse transcription real-time polymerase chain reaction Frederic R. Grau, Megan E. Schroeder, Erin L. Mulhern, Michael T. McIntosh, and Mangkey A. Bounpheng
10/12/2019, · For on-site ASFV ,detection,, lateral-flow strip readout was introduced for the first time in the RAA-Cas12a based system (named CORDS, Cas12a-based On-site and Rapid ,Detection, System). We used CORDS to detect target DNA highly specifically using the lateral-flow strip readout and the assay displayed no cross-reactivity to other 13 ,swine, viruses including ,classical swine fever, (CSF).
Introduction. ,African swine fever, (ASF) is a highly contagious hemorrhagic disease of domestic pigs and wild boars. ASF is caused by a large, complex double-stranded DNA virus, the ,African swine fever, virus (ASFV) (Galindo and Alonso, 2017).The disease shares similar clinical signs with ,classical swine fever, (CSF) and several other ,swine, diseases (Oura et al., 2013), making its diagnosis ...
17/6/2020, · Viral ,detection, is particularly important with a disease like ASF. ,African Swine Fever, has no pathognomonic signs. Put another way, the effects of ASF in pigs are similar to common diseases we face every day: PRRS or porcine circovirus, for example. The only way to definitively identify it is through directly detecting ASF virus in an animal.
The VetMAX ,African Swine Fever, Virus PCR test has been validated as part of an entire workflow for ASF ,detection,, from sample to answer including Applied Biosystems MagMAX CORE ,nucleic acid, extraction ,kits, and QuantStudio PCR instruments, and has proven useful in more than 1.5 million clinical ASF samples tested around the world.