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Nucleic acid, extraction involved either ,kit,-based or conventional phenol:chloroform:isoamyl alcohol methods. A single-round PCR assay, using primers that hybridize to the conserved p120 nonstructural gene region, was 82.5% sensitive (n = 17) and 100% specific (n = 18) in the ,detection, …
17/6/2020, · Viral ,detection, is particularly important with a disease like ASF. ,African Swine Fever, has no pathognomonic signs. Put another way, the effects of ASF in pigs are similar to common diseases we face every day: PRRS or porcine circovirus, for example. The only way to definitively identify it is through directly detecting ASF virus in an animal.
1/7/2020, · ,African swine fever, (ASF) is a highly contagious disease caused by ,African swine fever, virus (ASFV) in domestic pigs and wild boars, which results in huge economic losses to the pig industry. Because of its extremely infectious capacity, there is an urgent need to establish a convenient and low-cost diagnostic method for rapid and on-site ,detection, of the virus to timely implement the control ...
The VetMAX ,African Swine Fever, Virus PCR test has been validated as part of an entire workflow for ASF ,detection,, from sample to answer including Applied Biosystems MagMAX CORE ,nucleic acid, extraction ,kits, and QuantStudio PCR instruments, and has proven useful in more than 1.5 million clinical ASF samples tested around the world.
Classical Swine Fever, Volker Moennig 1, Paul Becher and Martin Beer2 ... •Virus (antigen, ,nucleic acid,) ,detection, •Immunohistochemistry •Virus isolation ... –,African swine fever, virus (ASFV) –Suid herpesvirus 1 (SuHV-1) –Foot-and-mouth disease virus (FMDV)
African swine fever,: ,detection, and diagnosis 9 789251 097526. Cover photographs: ... 18 Haemorrhages in a pig with ,classical swine fever, (CsF) 24. vi ... eDTA Ethylenediaminetetraacetic ,acid, eFSA European Food Safety Authority eLISA Enzyme-linked immunosorbent assay
Introduction. ,African swine fever, (ASF) is a highly contagious hemorrhagic disease of domestic pigs and wild boars. ASF is caused by a large, complex double-stranded DNA virus, the ,African swine fever, virus (ASFV) (Galindo and Alonso, 2017).The disease shares similar clinical signs with ,classical swine fever, (CSF) and several other ,swine, diseases (Oura et al., 2013), making its diagnosis ...
Classical swine fever, virus (CSFV) is the causa- tive agent of ,Classical swine fever, which is a highly contagious disease affecting ,swine, and resulting in severe economic losses. In this study, we developed reverse transcription loop- mediated isothermal amplification (RT-LAMP) assay targeting the 5’UTR gene for the ,detection, of CSFV.
Application of ,nucleic acid, based techniques for ,detection, of ,Classical swine fever, virus: A comparative study Elina Khatoon*1, Nagendra Nath Barman2, Manab Deka3, Gitika Rajbongshi4, Kongkon Baruah5, Nipu Deka6, Durlav Prasad Bora7 1 & 3 Department of Biotechnology, Gauhati University, 781014, Assam
2/10/2020, · The future fight against ,African swine fever, must even more focus on enhanced ,classical, control measures like early ,detection,, strict hygiene and biosecurity measures, culling of infected farms, compensation of losses, stand still measures, epidemiological tracing and wild boar control programs.